| Professor Phillip Butcher |
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Research interests
Philip Butcher is Professor of Molecular Medical Microbiology in the Centre for Infection at St George’s University of London. He is P.I. on The Wellcome Trust funded multi-collaborative microbial pathogen microarray project, based at St. George’s University of London – BuG@S. (http://bugs.sgul.ac.uk ) which provides microarrays for 12 bacterial pathogens as a resource to the bacterial research community. BuG@S has developed collaborations with over 80 different research groups, with at least 200 individual users of the facility in the UK and internationally.
Professor Butcher’s other research interests include the study of gene expression in Mycobacterium tuberculosis during interaction with host cells such as macrophages and dendritic cells (Schnappinger et al J.Exp.Med 2004; Tailleux et al PLoS One 2008) and directly from human sputum (Garton et al PLoS Medicine 2008) revealing new insights into the metabolic adaptations of intracellular and in vivo bacteria which may explain the basis of drug tolerance and thus the need for prolonged treatment regimes for TB. Professor Butcher is a partner in the EU FP6 funded consortium called New Medicines for Tuberculosis (http://www.nm4tb.org/) and provides microarray expertise as enabling technologies in the discovery of new drug targets and anti-TB drugs (Makarov et al Science 2009).
The other area of Professor Butcher’s research activity lies in the development of microarray-based diagnostics for the simultaneous detection of multiple pathogens (Boriskin et al J Clin Microbiol 2004) in CNS infections and more recently for sexually transmitted infections, funded by NHS R&D, OGT Ltd and The Heptagon Fund. This has led to the development of a UKCRC/MRC funded multi-disciplinary and multi-Institutional consortium based at St George’s (eSTI2) that aims to develop and evaluate point-of-care diagnostics for STIs linked to telecommunication technology.
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Publications
Makarov, V. et al (2009). Benzothiazinones Kill Mycobacterium tuberculosis by Blocking Arabinan Synthesis. Science (2009) 324:801-804. (10.1126/science.1171583) (published online 19/03/09, PMID:19299584)
Stavrum R, Valvatne H, Bø TH, Jonassen I, Hinds J, Butcher PD, and Grewal HM (2008) Genomic diversity among Beijing and non-Beijing Mycobacterium tuberculosis isolates from Myanmar. PLoS ONE 3(4):e1973
Waddell SJ, Laing K, Senner C, Butcher PD (2008) Microarray analysis of defined Mycobacterium tuberculosis populations using RNA amplification strategies. BMC Genomics. 2008 Feb 25;9(1):94
Tailleux L, Waddell SJ, Pelizzola M, Mortellaro A, Withers M, Tanne A, Ricciardi-Castagnoli P, Gicquel B, Stoker NG, Butcher PD, Foti M & Neyrolles O. (2008) Probing Host Pathogen Cross-talk by Transcriptional Profiling of Both Mycobacterium tuberculosis and Infected Human Dendritic Cells and Macrophages. PLoS ONE. 2008 Jan 2;3(1):e1403.
Bacon J, Dover LG, Hatch KA, Zhang Y, Gomes JM, Kendall S, Wernisch L, Stoker NG, Butcher PD, Besra GS, Marsh PD (2007) The lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture: identification of a novel wax ester Microbiology. May;153(Pt 5):1435-44.
Brodin P , Majlessi L, Marsollier L, de Jonge MI, Bottai D, Demangel C, Hinds J, Neyrolles O, Butcher PD, Leclerc C, Cole ST and Brosch R (2006) Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. Infect Immun. 74(1):88-98
Witney AA, Marsden GL, Holden MTG, Stabler RA, Husain SE, Vass JK, Butcher PD, Hinds J and Lindsay JA. (2005) Design, validation and application of a seven-strain Staphylococcus aureus PCR product microarray for comparative genomics. Appl.Environ.Micrbiol. 71(11):7504-7514
Boriskin YS, Rice PS, Stabler RA, Hinds J, Al-Ghusein H, Vass K and Butcher PD. (2004) DNA microarrays for virus detection in CNS infections. J.Clin.Microbiol. 42: 5811-5818
Waddell SJ, Chung GA, Gibson KJC, Everett MJ, Minnikin DE, Besra GS and Butcher PD. Inactivation of polyketide synthase and related genes results in the loss of complex lipids in Mycobacterium tuberculosis H37Rv. Letters in Applied Microbiology. 2005;40(3):201-206
Rajakumar K, Shafi J, Smith RJ, Stabler RA, Andrew PW, Modha D, Bryant G, Monk P, Hinds J, Butcher PD, Barer MR. (2004). Use of genome level-informed PCR as a new investigational approach for analysis of outbreak-associated Mycobacterium tuberculosis isolates. J Clin Microbiol. 2004; 42(5):1890-6
Bacon J, James BW, Wernisch L, Williams A, Morley KA, Hatch GJ, Mangan JA, Hinds J, Stoker NG, Butcher PD and Marsh PD (2004) The influence of reduced oxygen availability on pathogenicity and gene expression in M. tuberculosis. Tuberculosis (Edinb). 2004;84(3-4): 205-17
Hampshire T, Soneji S, Bacon J, James BW, Hinds J, Laing K, Stabler RA, Marsh PD and Butcher PD (2004) Stationary phase gene expression of Mycobacterium tuberculosis following a progressive nutrient depletion: a model for persistent organisms. Tuberculosis (Edinb). 2004;84(3-4):228-38
Butcher PD (2004). Microarrays for Mycobacterium tuberculosis. Tuberculosis (Edinb). 2004;84(3-4):131-137
Li M-S, Waddell SJ, Monahan IM, Mangan JA, Martin SL, Everett MJ and Butcher PD. (2004) Increased transcription of a potential sigma factor regulatory gene Rv1364c in Mycobacterium bovis BCG while residing in macrophages indicates use of alternative promoters. FEMS Microbiology Letters 233(2):333-339
Kutalik Z, Inwald J, Gordon SV, Hewinson RG, Butcher P, Hinds J, Cho K-H, Wolkenhauer O (2004) Advanced significance analysis of microarray data based on weighted resampling: a comparative study and applications to gene deletions in Mycobacterium bovis. Bioinformatics 20(3):357-363
Schnappinger D, Ehrt S, Voskuil M, Liu Y, Mangan JA, Monahan IM, Dolganov G, Efron B, Butcher PD, Nathan C & Schoolnik GK (2003) Transcriptional adaptation of Mycobacterium tuberculosis within macrophages: insights into the phagosomal environment. J.Exp.Med. 198:693-704
Attia MA, Welsh JP, Laing K, Butcher PD, Gibson FM, Rutherford TR.(2003) Fidelity and reproducibility of antisense RNA amplification for the study of gene expression in human CD34+ haemopoietic stem and progenitor cells. Br J Haematol. 122(3):498-505
Wernisch L, Kendall SL, Soneji S, Wietzorrek A, Parish T, Hinds J, Butcher PD, Stoker NG. (2003) Analysis of whole-genome microarray replicates using mixed models. Bioinformatics. 19(1)53-61
Mangan,JA., I.M. Monahan and P.D. Butcher (2002) Gene expression during host-pathogen interactions: approaches to bacterial mRNA extraction and labelling for microarray analysis, p137-151. In: B.W. Wren and N. Dorrell (eds.), Methods in Microbiology vol 33: Functional Microbial Genomics. Academic Press, London
Butcher PD (2002) Bacterial pathogens, microarrays and functional genomics Comparative and Functional Genomics 3(4), 326-329
Stewart GR, Wernisch L, Stabler R, Mangan J, Hinds J, Laing KG, Young DB, Butcher PD. (2002) Dissection of the heat-shock response in Mycobacterium tuberculosis using mutants and microarrays. Microbiology. 148, 3129-3138
Li M-S, Monahan IM, Waddell S, Mangan JA, Everettt MJ, Martin S, Butcher PD. (2001) cDNA - RNA subtractive hybridization reveals increased expression of mycocerosic acid synthase in intracellular Mycobacterium bovis BCG Microbiology 147: 2293-2305
Monahan IM, Betts J, Banerjee DK and Butcher PD (2001) Differential expression of mycobacterial proteins following phagocytosis by macrophages. Microbiology 147, 459-471
Hu Y-M, , Mangan JA, Dhillon J, Sole KM, Mitchison DA, Butcher PD, Coates ARM. (2000) Detection of mRNA transcripts and active transcription in persistent Mycobacterium tuberculosis induced by exposure to rifampin and pyrazinamide. J.Bacteriol. 182 6358-6365
Eltringham IJ, Drobniewski FA, Mangan JA, Butcher PD, Wilson SM (1999) Evaluation of RT-PCR and a bacteriophage based assay for the rapid phenotypic detection of rifampin resistance in clinical isolates of Mycobacterium tuberculosis. J.Clinical Microbiology 37, 3524-3527
Agranoff D, Monahan IM, Mangan JA, Butcher PD, Krishna S. (1999) Mycobacterium tuberculosis expresses a novel pH-dependent divalent cation transporter belonging to the Nramp family. J.Exp.Med. 190, 1-9
Hu Y-M, Butcher PD, Mangan JA, Rajandream M-A, Coates ARM. (1999) Regulation of hmp gene transcription in Mycobacterium tuberculosis: effects of oxygen limitation and nitrosative and oxidative stress J.Bacteriol. 181, 3486-3493
Butcher PD, Sole KM, Mangan JA (1998) RNA extraction. In: Molecular Mycobacteriology: techniques and clinical applications. (Eds. Ollar RA & Connell ND) Chapter 14. Marcel Dekker Inc, USA
Butcher PD, Mangan J, Monahan IM (1998) Intracellular gene expression: analysis of RNA from mycobacteria in macrophages using RT-PCR. Methods in Molecular Biology volume 101: Mycobacteria Protocols, 285-306. (eds. Parish T & Stoker NG). Humana Press Inc., USA
Hu Y-M, Butcher PD, Sole K, Mitchison DA, Coates ARM (1998) Protein synthesis is shutdown in dormant Mycobacterium tuberculosis and is reversed by oxygen or heat shock. FEMS Microbiol.Lett. 158, 139-145
Mangan JA, Sole KM, Mitchison DA, Butcher PD (1997) An effective method of DNA extraction from bacteria refractory to disruption, including mycobacteria. Nucleic Acids Res. 25(3), 675-677 |
